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FIGURE 5 Cytotoxic UVB effect on the HaCaT <t>keratinocyte</t> cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).
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FIGURE 5 Cytotoxic UVB effect on the HaCaT keratinocyte cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).

Journal: Photochemistry and photobiology

Article Title: Full-spectrum cannabidiol reduces UVB damage through the inhibition of TGF-β1 and the NLRP3 inflammasome.

doi: 10.1111/php.13993

Figure Lengend Snippet: FIGURE 5 Cytotoxic UVB effect on the HaCaT keratinocyte cell line. (A) The percentage of surviving cells decreased significantly after UVB irradiation was applied from 360 to 5760 mJ/cm2, MTT reduction (***p < 0.0001 vs. 0, one-way ANOVA with Tukey's test). (B) The LD50 was 1247 mJ/cm2 according to the log (inhibitor) versus response, variable slope (four parameters) statistical test. (C) The normality of the data was evaluated using a normal QQ plot: normality and lognormality test (alpha = 0.05).

Article Snippet: Human skin- derived keratinocytes (HaCaT cells; American Type Culture Collection (ATCC PCS200- 011) Manassas, VA) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS; Gibco) and a 1% antibiotic–antimycotic solution (Gibco).

Techniques: Irradiation

FIGURE 7 Photoprotective effect of FS-CBD on UVB irradiation-induced damage in the HaCaT keratinocyte cell line. The percentage of viable cells was evaluated after exposure to 0, 1.5, and 3 μg/mL FS-CBD and irradiation with UVB (1080, 1620, and 2160 mJ/cm2). FS-CBD protected against UVB-induced cell death, and viability was evaluated using MTT assay (&&&p < 0.0001 vs. all groups, ωωωp < 0.0001 vs. the 0, 1080 mJ/cm2 group, ψψψ p < 0.0001 vs. the 0, 1620 mJ/cm2 group, *** p < 0.0001 vs. the 0, 2160 mJ/cm2 group, one-way ANOVA with Tukey's test).

Journal: Photochemistry and photobiology

Article Title: Full-spectrum cannabidiol reduces UVB damage through the inhibition of TGF-β1 and the NLRP3 inflammasome.

doi: 10.1111/php.13993

Figure Lengend Snippet: FIGURE 7 Photoprotective effect of FS-CBD on UVB irradiation-induced damage in the HaCaT keratinocyte cell line. The percentage of viable cells was evaluated after exposure to 0, 1.5, and 3 μg/mL FS-CBD and irradiation with UVB (1080, 1620, and 2160 mJ/cm2). FS-CBD protected against UVB-induced cell death, and viability was evaluated using MTT assay (&&&p < 0.0001 vs. all groups, ωωωp < 0.0001 vs. the 0, 1080 mJ/cm2 group, ψψψ p < 0.0001 vs. the 0, 1620 mJ/cm2 group, *** p < 0.0001 vs. the 0, 2160 mJ/cm2 group, one-way ANOVA with Tukey's test).

Article Snippet: Human skin- derived keratinocytes (HaCaT cells; American Type Culture Collection (ATCC PCS200- 011) Manassas, VA) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS; Gibco) and a 1% antibiotic–antimycotic solution (Gibco).

Techniques: Irradiation, MTT Assay